Average Nucleotide Identity (ANI)FastANI tool: to compare draft and reference genomes Jain, C., Rodriguez-R, L.M., Phillippy, A.M. et al., Nature Communication (2018). -r <value>, --ref <value> reference genome (fasta/fastq)[.gz] -q <value>, --query <value> query genome (fasta/fastq)[.gz] °°° fastANI -q draft_genome.fasta -r reference_genome.fasta -o fastani.out cat fastani.out draft_genome.fasta reference_genome.fasta 96.41 330 352 Mapping result Nucleotide Identity (ANI) = 96.41 330 of total 352 sequence fragments were aligned as orthologous matches |
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