Basic Local Alignment Search Tool (BLAST)BLAST is a software tool for searching similarity in nucleotide sequences (DNA) and/or amino acid (protein) sequences.
Blast FAQ
https://blast.ncbi.nlm.nih.gov/blast/FAQOptions
Blast results
Blast database makeblastdb -in ecoli.fasta -parse_seqids -dbtype nucl → Bowtie2 to map short reads (shotgun sequencing) against reference genomes → FastANI to compare (draft) genomes by Average Nucleotide Identity (ANI) |
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