Measures for community richness within an environment or variation between different environments Assembly Aligning and merging short fragments of sequenced DNA in order to reconstruct the original genome. Coverage depth Average number of times a base of a genome is sequenced. Contiguous fragments of DNA sequence from an incomplete draft genome. GC content The GC content of a DNA sequence is the percentage of nucleotides that are either G or C. Giga base pairs (Gb) Size of a metagenomics sample in numbers of base pairs Horizontal gene transfer (HGT) Exchange or absorption of genetic material independent of reproduction Environmental gene tags (EGTs) Short DNA sequences that characterize microbial environments. Collection of biological DNA fragments prepared for sequencing A microbial ecosystem is the community of all microbes living in a specific environment Identifying the complete set of transcripts (RNA-seq) from microbial environments Metaproteomics profiling of community-wide protein abundances Multilocus sequence typing (MLST) Technique to detect variability of housekeeping genes for identifying bacterial strains DNA fragments from different samples are pooled and sequenced all together Sequence based species cluster defined by 16S gene sequence similarity Orthologous genes Functional identical genes in different species that evolved from a common ancestral gene The entire gene set of a species Quality value for each sequenced base Ribosomal sequences 16S To identify and compare bacteria based on differences in their 16S ribosomal sequence Sampling depth How deep is enough for metagenomic shotgun sequencing Strain-level metagenomics Identifying the gene composition of individual strains in metagenomic samples |
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